Figure 1.

(A) Workflow on how to adhere cells suitable for LigandTracer assays. Target and control cells are adhered in defined areas opposite each other on a Petri dish with the help of biomolecular anchor molecule (BAM). (B) Measurement principle of LigandTracer. The dish containing target and control cells is placed on an inclined and rotating support. A detector is mounted above the upper part of the dish. Cell medium with labeled ligand is added to the dish and due to the inclination of the dish remains in its lower part during the measurement. The fluorescence intensity from both target and control cells is measured once per rotation in the upper position.