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. 2005 Jan;12(1):44–51. doi: 10.1128/CDLI.12.1.44-51.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 1. — One-dimensional (A) and two-dimensional TLC analysis (B to D) of GPLs and GPL core. Three reference strains of MAC were analyzed: serotypes 4 (ATCC 35767, lane 1), 16 (ATCC 13950, lane 2), and 20 (ATCC 35764, lane 3). GPLs were purified repeatedly by one-dimensional (1D) TLC developed with chloroform-methanol-distilled water until a single spot was obtained (A). Subsequently GPLs were β-eliminated to obtain GPL core, and then the purity of GPL core was examined by two-dimensional (2D) TLC (first in the vertical and second in the horizontal direction. B, serotype 4; C, serotype 16; D, serotype 20).