Fig. 1.

A: PBMCs were cultured in the specified growth factor cocktail for 6 days. Nonadherent cells collected from culture plates were classified as multipotent (MPP), erythroid (MEP), and nonerythroid (NE) progenitors based on a combination of cell surface antigens. First, lineage-negative (lin^neg), CD34⁺, and CD38⁺ cells (lin^negCD34⁺CD38⁺) were selected for further characterization. The lin^negCD34⁺CD38⁺ cells comprised both MafB⁺ and MafB^neg cells, which confirms their multipotency (MPPs are the solid line in the histogram and the dashed line is Ab control). Furthermore, MPPs were subdivided according to differential expressions of CD123 (IL-3Rα) and CD45RA, such that MEPs are gated as CD123^neg CD45RA^neg and N-E cells as CD123^+/− CD45RA⁺ and verified with MafB⁺ (N-E) and MafB^neg (MEP) expressions. GMCSF, granulocyte macrophage colony-stimulating factor; SCF, stem cell factor. B: representative images of PBMC-derived MEPs and MafB-expressing cells in MPP population from standard burn care (SBC) patient (E02) at post burn day 7 (PBD7) (<2 wk) and PBD35 (4–7 wk); propranolol (PR)-treated patient (E05) at PBD9 (<2 wk) and PBD38 (4–7 wk).