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. 2016 Sep 1;122(3):620–630. doi: 10.1152/japplphysiol.00632.2016

Table 3.

Primer sequences used for real-time PCR (previously unpublished)

Primer Sequence (5′-3′)
mRNA Target Accession No. Forward Reverse Product Size, bp
ATG7 NM_006395 GCTCTTCCTTACTTCTTA ATTGTTATCTTCGTCCTT 104
BECN1 NM_003766 GATACCGACTTGTTCCTTACG TATAACGGCAGCTCCTTAGAT 76
BNIP3 NM_004052 CATCGGATTGGGGATCTATATT ATCCACTAACGAACCAAGTC 94
GABARAP NM_007278 TGCCGGTGATAGTAGAAAAG GTAGAACTGACCAACTGTGA 98
LAMP2B NM_013995 GTATTCTACAGCCCAAGAGTGTT AATCAAGCCTGAAAGACCAGC 85
LC3 NM_022818 CCGCACCTTCGAACAAAGAG AAGCTGCTTCTCACCCTTGT 103
CISD2 NM_001008388 TTCGCTAGGCTCACAGTTTCA TCTTCGGGAGGAATGGACGA 100
RUNX1 NM_001001890 CTTTCAAGGTGGTGGCCCTA CATGGCTGCGGTAGCATTTC 110
Fn14 NM_016639 GAAGTTCACCACCCCCATA GATGAATGAATGATGAGTGGG 116
TRAF6 NM_145803 GGTCCGGAATTTCCAGGAAA CATTTTAGCAGTCAGCTCCCG 88

ATG7, autophagy related 7; BECN1, Beclin-1; BNIP3, BCL2/adenovirus E1B 19-kDa interacting protein 3; GABARAP, GABA(A) receptor-associated protein; LAMP2B, lysosomal-associated membrane protein 2 transcript variant B; LC3, microtubule associated protein 1 light chain 3; CISD2, CDGSH iron-sulfur domain-containing protein 2; RUNX1, runt-related transcription factor 1; Fn14, fibroblast growth factor-inducible 14; TRAF6, tumor necrosis factor receptor-associated factor 6.