Table 3.
Primer Sequence (5′-3′) |
||||
---|---|---|---|---|
mRNA Target | Accession No. | Forward | Reverse | Product Size, bp |
ATG7 | NM_006395 | GCTCTTCCTTACTTCTTA | ATTGTTATCTTCGTCCTT | 104 |
BECN1 | NM_003766 | GATACCGACTTGTTCCTTACG | TATAACGGCAGCTCCTTAGAT | 76 |
BNIP3 | NM_004052 | CATCGGATTGGGGATCTATATT | ATCCACTAACGAACCAAGTC | 94 |
GABARAP | NM_007278 | TGCCGGTGATAGTAGAAAAG | GTAGAACTGACCAACTGTGA | 98 |
LAMP2B | NM_013995 | GTATTCTACAGCCCAAGAGTGTT | AATCAAGCCTGAAAGACCAGC | 85 |
LC3 | NM_022818 | CCGCACCTTCGAACAAAGAG | AAGCTGCTTCTCACCCTTGT | 103 |
CISD2 | NM_001008388 | TTCGCTAGGCTCACAGTTTCA | TCTTCGGGAGGAATGGACGA | 100 |
RUNX1 | NM_001001890 | CTTTCAAGGTGGTGGCCCTA | CATGGCTGCGGTAGCATTTC | 110 |
Fn14 | NM_016639 | GAAGTTCACCACCCCCATA | GATGAATGAATGATGAGTGGG | 116 |
TRAF6 | NM_145803 | GGTCCGGAATTTCCAGGAAA | CATTTTAGCAGTCAGCTCCCG | 88 |
ATG7, autophagy related 7; BECN1, Beclin-1; BNIP3, BCL2/adenovirus E1B 19-kDa interacting protein 3; GABARAP, GABA(A) receptor-associated protein; LAMP2B, lysosomal-associated membrane protein 2 transcript variant B; LC3, microtubule associated protein 1 light chain 3; CISD2, CDGSH iron-sulfur domain-containing protein 2; RUNX1, runt-related transcription factor 1; Fn14, fibroblast growth factor-inducible 14; TRAF6, tumor necrosis factor receptor-associated factor 6.