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Indirect protein ELISA of MAb 12C6 with 140-kDa AAP. A 96-well ELISA plate was coated with AAP. MAb 12C6 was added to each well and incubated for 3 h at 37°C. Bound MAb were detected with alkaline phosphatase-conjugated goat anti-mouse IgG. □, MAb 12C6; ▪, MAb 1934 (an irrelevant MAb against fibronectin).
