Fig. 7.

FTY720 does not directly alter hepatic stellate cell or macrophage activation. The hepatic stellate cell line (LX-2) was treated with 10 ng/ml TGFβ for 1 h followed by 250 nM FTY720 for 4 h. The expression of markers of hepatic stellate cell activation α-smooth muscle actin (αSMA) (A), Col1α₁ (B), connective tissue growth factor (CTGF; C), platelet-derived growth factor-bb (PDGF-BB) (D), and proinflammatory genes interleukin 1 beta (IL1β) (E) and monocyte chemotactic protein 1 (MCP-1; F) was measured by quantitative PCR. BMDMs were treated with 50 ng/ml LPS for 1 h followed by 250 nM FTY720 for 4 h. The expression of proinflammatory genes Mcp-1 (G), and IL-1β (H), and the profibrotic gene platelet-derived growth factor-bb (PDGF-BB) (I) were measured by qPCR. αSMA, Col1α₁, and CTGF were not detected in BMDM. J: cell death in LX-2 cells stimulated with TGFβ and treated with FTY720 as above. K: cell death in BMDM stimulated with LPS and treated with FTY720 as above. L: cell death in primary mouse hepatocytes treated with 250 nM FTY720 for 16 h. *P < 0.05.