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FFC-feeding increases circulating extracellular vesicles (EVs) and their sphingolipid content. A: circulating EVs were measured in 200 μl of plasma obtained from each experimental group. EVs were quantified by nanoparticle tracking analysis. *P < 0.05, **P < 0.01, ***P < 0.001. B: circulating EV S1P content and C16:0 ceramide content was calculated as the product of the EV particle concentration and S1P (ng/EV) and C16:0 (ng/EV), respectively. *P < 0.05.
