Table 1.

Sequences of primers used in this study

Primer Name	Use	Primer Sequence (5′–3′)
HIF1-Forward	PCR	AGGAGGGGGAAGGAATCTGAGGT
HIF1-Reverse	PCR	ACAACACACTGGGGCTGGGAG
AP-1	RACE	CCATCCTAATACGACTCACTATAGGGC
AP-2	RACE	ACTCACTATAGGGCTCGAGCGGC
HIF1 5′gs outer	RACE	GTCTCCGTCTTCAGACAGCACCAG
HIF1 5′gs inner	RACE	AGCTGCATGTCCAGAGCTGGTTTG
HIF1 5′gs alt	RACE	CTCTAAAGCCTTCAGGTAGGAGCC
HIF1 3′gs outer	RACE	CGGAGGACCTGCTGAACCGGTCTG
HIF1 3′gs inner	RACE	CAAAGACTCACCATCACTTGTTTG
HIF1 3′gs alt	RACE	ACCACGCTCTGGACTCGGACTATC
HIF3 5′gs outer	RACE	ACAGGGGTTCGGTTTTGGGTAG
HIF3 5′gs inner	RACE	TGTTGTTGTAGAGGACGGTGGC
HIF3 3′gs outer	RACE	TGACCTTCCTCAGCGATTTGC
HIF3 3′gs inner	RACE	AAGCAAGAGCCGTCCTCCATAG
HIF1 5′UTR	PCR	GAACCCAGGAGGAACTCTTATGTG
HIF1 3′UTR	PCR	TAAATAAGTGGCAGTGGGGTC
HIF3 5′UTR	PCR	AACAGAGAGCCTTGGATTTGGTGTTC
HIF3 3′UTR	PCR	GGCGAGGATCTTTTGTGCGTACAG
HIF2 5′subclone	Ab production	TTCACTGCAGAGCCCAGGTGATTACTACAGC
HIF2 3′subclone	Ab production	TTCAGTCGACCCTCTGTCGGGTGTCCACAG
HIF3 5′subclone	Ab production	TTCACTGCAGTTCCAGCTGACCTTCCTCAGC
HIF3 3′subclone	Ab production	TTCAGTCGACGAAGATGTCCCTCATCAGAGCTG
HIF1-F1	QPCR	CAAGTCGGCTACGTGGAAGGTG
HIF1-R1	QPCR	CAGATCAGGACCAGATAGGGGAC
HIF2-F1	QPCR	GGCTTCATCACCGTGGTAACATC
HIF2-R1	QPCR	CTGTGACCTGTGAGCTCCACCTG
HIF3-F1	QPCR	GTCAACAAGCACATCGGCATCACG
HIF3-R1	QPCR	CCATCAGTTTCTTACTCAGACCTGG

Italicized bases indicate restriction enzyme sites included for subcloning.