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. 2016 Dec 6;312(3):H515–H527. doi: 10.1152/ajpheart.00499.2016

Fig. 1.

Fig. 1.

Treatment with low glucose (LG) elicits a mitochondrial fission response. A: LG induced mitochondrial fragmentation in endothelial cells and inhibition of dynamin-related protein 1 (Drp1) activity with Mdivi-1 (10 μM) attenuated the response (P = 0.003 overall, *P < 0.02 for LG vs. NG and LG + Mdivi-1, N = 6). Scale bars represent 10 μm. B: similarly, when expression of Drp1 was decreased via siRNA knockdown, mitochondrial fragmentation was decreased in LG-exposed cells (P < 0.001 overall, *P = 0.025 for LG + Scramble vs. LG + siDrp1 and NG, N = 6). Scale bars represent 10 μm. C: Human umbilical vein endothelial cells (HUVECs) exposed to siRNA constructs against Drp1 displayed ~50% decrease in protein expression when analyzed by Western blot (*P < 0.05, N = 6). D: proof of concept in translating cellular knockdown of Drp1 expression to our ex vivo preparation of human tissues. Vessels were exposed to siDrp1 constructs per protocol and then analyzed for Drp1 expression via immunohistochemistry. The endothelium of samples exposed to siDrp1 displayed a decrease in Drp1 expression when compared with scrambled control (arrows). Scale bars represent 10 μm.