Fig. 3.

Increased NO bioavailability in human arterioles with pharmacologic inhibition of fission. Human arterioles were incubated in either NG or LG buffer with or without fission inhibitor, Mdivi-1 (10 µmol/l). NO was visualized with the fluorescent NO sensitive fluorescent probe DAF2-DA. LG treatment reduced NO presence in isolated microvessels, and blockade of the fission process with Mdivi-1 increased NO levels in vessels exposed to LG (P < 0.001 overall, *P < 0.05 for LG vs. NG and LG+Mdivi-1, N = 4). Scale bars represent 50 μm.