Fig. 4.

Knockdown of IFT88/Polaris prevents ciliogensis in cultured ECs. A: Western blot images showing effect depletion of IFT88 protein levels in ECs transfected with IFT88 siRNA compared with negative control siRNA. B: quantification of immunofluorescent images showing number of ciliated cells 72 h following transfection. C: maximum projection of a confocal z-stack shows control siRNA-treated ECs stained for primary cilia extending from the apical surface marked by acetylated α-tubulin (green), and DNA is stained with DAPI (blue). D: maximum projection of IFT88 siRNA-transfected cells showing the absence of primary cilia on the cell surface as marked by acetylated α-tubulin staining (n = 3). Values are means ± SE. ****P < 0.0001 vs. control.