Fig. 5.

Primary cilia are not required for the FSS induction of COX-2 in ECs. A: Western blots showing FSS induction COX-2 protein expression in BAECs occurred in the presence and absence of primary cilia. BAECs were exposed to 10 dyn/cm2 of FSS for 5 h post 72 h of transfection with IFT88 siRNA. B: densitometric analysis of Western blots showing depletion of primary cilia had no significant inhibitory effect on the FSS induction of COX-2 in BAECs. Densitometric analysis was accomplished by dividing the target protein densitometric units by β-actin (loading control) densitometric units to normalize for loading, and then normalizing by the control siRNA-treated controls. Values are means ± SE. *P < 0.05 vs. control siRNA or static (S) controls.