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. 2016 Dec 23;312(3):H485–H500. doi: 10.1152/ajpheart.00035.2016

Fig. 6.

Fig. 6.

Knockdown of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression inhibits COX-2 protein expression in BAECs and human umbilical vein endothelial cells (HUVECs). A: Western blots showing the expression of PECAM-1 after treatment with control and PECAM-1 siRNA in BAECs (n = 3). B: Western blots showing COX-2 expression in BAECs treated with control or PECAM-1 siRNA for 72 h before exposure to FSS for 5 h (n = 4). C: Western blots showing the expression of PECAM-1 after treatment with control and PECAM-1 siRNA, compared with HUVECs treated with control siRNA (n = 3). D: COX-2 expression in HUVECs treated with control or PECAM-1 siRNA for 72 h before exposure to FSS for 5 h (n = 4) compared with controls. Densitometric analysis was accomplished by dividing the target protein densitometric units by β-actin (loading control) densitometric units to normalize for loading and then normalizing by the control siRNA-treated controls. Values are means ± SE. *P < 0.05 vs. untreated static (S) controls. #P < 0.05 vs. untreated flow (F) controls.