Table 1.

Arachidonic acid metabolites were measured by LC-MS

	SD Sham	SD RI	SD RI + miR-145	SD RI + Anti-miR-145	JCR Sham	JCR RI	JCR RI + miR-145	JCR RI + Anti-miR-145	JCR RI + Block Ab
20-HETE	7.2 ± 3	19.5 ± 5†	5.0 ± 1*	39.6 ± 2*†	24.3 ± 4*	50.7 ± 5*†	22.5 ± 4‡	74.4 ± 3*†‡	24.8 ± 6*‡
15-HETE	216.2 ± 2	211.3 ± 2	186.8 ± 6	266.8 ± 3*	312.3 ± 8*	513.8 ± 4*†	108.6 ± 8‡	523.4 ± 9*†	519.5 ± 2*†
12-HETE	1098.1 ± 3	1114.1 ± 4	1000.6 ± 4	1564.3 ± 5*	5173.5 ± 12*	7232.8 ± 11*	2225.9 ± 8*‡	7251.6 ± 12*	7239.2 ± 9*
5-HETE	3.8 ± 2	4.2 ± 2	4.1 ± 3	14.4 ± 5*	158.7 ± 8*	208.4 ± 6*	111.2 ± 7*‡	206.4 ± 3*	211.4 ± 1*
14(15)-EET	299.0 ± 8	304.4 ± 6	301.4 ± 6	303.4 ± 2	139.3 ± 8*	79.3 ± 5*	80.4 ± 3*	78.2 ± 4*	81.3 ± 1*
11(12)-EET	634.0 ± 6	629.6 ± 9	641.1 ± 9	622.2 ± 12	101.3 ± 6*	55.2 ± 8*†	56.6 ± 2*†	57.6 ± 4*†	55.6 ± 4*†
8(9)-EET	101.1 ± 4	89.6 ± 7	108.5 ± 2	103.5 ± 7	102.5 ± 9	81.4 ± 4	85.3 ± 9	85.4 ± 3	83.0 ± 3
5(6)-EET	245.6 ± 11	286.2 ± 6	249.2 ± 8	273.248	275.3 ± 3	183.7 ± 5*	190.1 ± 9*	192.7 ± 11*	181.2 ± 5*

Values are means ± SE in pg/mg. Concentrations in pg/mg tissue in the collateral-dependent zone (CZ) are shown. LC-MS, liquid chromatography-mass spectrometry; SD, Sprague Dawley; NZ, normal zone; RI, repetitive ischemia.

^*P < 0.05 vs. SD.

^†P < 0.05 vs. day 0 RI.

^‡P < 0.05 vs. JCR.