FIG. 1.

OPN production by human monocytes and immature DCs. Human CD14⁺ monocytes (Mo) were incubated for 5 days in the presence and absence of GM-CSF (800 U/ml) and IL-4 (500 U/ml). Twenty-four, 72, and 120 h after incubation, OPN in the culture supernatant was quantified with an EIA kit. OPN production by monocyte-derived maturing DCs increased during the culture period, whereas production by monocytes did not change. Data are shown as the means ± standard errors of the means (error bars) from three independent experiments.