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. 2017 Mar 30;3(2):105–114. doi: 10.1002/cjp2.65

Figure 1.

Figure 1

Validation of the anti‐CCR7 antibody. Western blotting showing specific antigen‐binding of the anti‐CCR7 primary antibody in the cell line lysates of: MCF7, lane 1, SKBR3, lane 2, MDA‐MB‐231, lane 3, MDA‐MB‐468, lane 4, HeLa, lane 5 and MCF10A, lane 6. (A) CCR7 protein at the expected molecular weight of 42.87 kDa, visualized in lanes 1–6 as green fluorescent bands in the 800 channel of the Odyssey Imaging System. (B) β‐actin protein at the expected molecular weight of 42 kDa visualized in lanes 1–6 as red fluorescent bands in the 700 channel of Odyssey Imaging System. (C) Multiplexed image of both anti‐rabbit and anti‐mouse secondary antibodies used in (A) and (B). The yellow bands indicate the overlapping proteins with molecular weight around 42 kDa. The molecular weight marker has both red and blue colored bands.