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. 2017 Apr 9;2017:8317913. doi: 10.1155/2017/8317913

Figure 5.

Figure 5

Rapamycin inhibits the cell proliferation and induced apoptosis via suppression of the mTOR/p70S6K signaling pathway in EC9706 cells. EC9706 cells were treated with 100 nmol/l rapamycin for 24 h. (a) Cell viability was examined by MTT assay. (b) Western blot analysis of Ki-67 expression. (c) Apoptosis rates in EC9706 cells were assessed by flow cytometry. (d) Western blot analysis of Bcl-2 and Bax expression. (e) qRT-PCR was used to determine the expressions of miR-195 and HMGA2 mRNA. (f) Western blot analysis of HMGA2 protein expression. β-actin was used as an internal control. Values are presented as the mean ± SD, n = 3. ∗∗P < 0.01, ∗∗∗P < 0.001.