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Pseudo-NORs are transcriptionally inactive. Ongoing pol I-mediated transcription was visualized in clones 5A, 4E, 5B, and 5E by incorporation of BrUTP into nascent transcripts. (Left panels) BrUTP was visualized with anti-BrdUTP antibodies (Roche) combined with FITC-conjugated secondary antibodies. (Center panels) UBF was detected using affinity-purified antibodies conjugated to rhodamine. Right panels show merged fluorescence and DIC images of the cells. Arrows depict pseudo-NORs.
