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. 2017 Apr 24;7:46715. doi: 10.1038/srep46715

Figure 7. Role of ABCB4 and ABCG1 in PE relocalisation and osteoclast formation.

Figure 7

(A) Gene expressions of Abcb4 and Abcg1 during osteoclastogenesis as analysed by quantitative PCR (n = 3 to 5). (B–F) shRNA-mediated knockdown of ABCB4 and ABCG1 in osteoclasts. Osteoclast precursors were infected with retroviruses expressing shRNAs for ABCB4 and ABCG1 (alone or in combination) or control and then cultured. (B) Efficiency of shRNA-mediated knockdown was validated by quantitative PCR (n = 10). (C) The shRNA retrovirus-infected cells were treated with SA-Bio-Ro, immunostained, and quantified the fluorescence intensity (n = 10 to 12). Representative fluorescence images are shown in (D). (E) The shRNA retrovirus-infected cells were stained for TRAP, and TRAP-positive multinucleated osteoclasts were counted (n = 12). (F) Expressions of osteoclast-related genes in the shRNA retrovirus-infected cells (n = 6). Each value is the mean ± SE.