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. 2005 Jan 1;19(1):77–89. doi: 10.1101/gad.1265105

Figure 1.

Figure 1.

Characterization of dicer-related genes. (A) Comparison of RNase III enzymes. Three classes of RNase III proteins, Escherichia coli RNase III-like, Drosha-like, and Dicer-like are schematically drawn. N termini of Dcr1p and Dcr2p (dotted lines) have not been determined experimentally. (B) mRNA expression of dicer-related genes analyzed on Northern blot. (g) Log-phase growing cells; (s) starved cells; (2–20) conjugating cells (numbers indicate hours post-mixing). Gene-specific probes were used for hybridization. Hybridization to a probe for rpL21, encoding a ribosomal protein was used as a loading control. (C) DCL1 mRNA is expressed in vegetative cells. Total RNA extracted from log-phase growing wild-type B2086 (lane 1), CU428 (lane 2), and mating B2086 and CU428 cells at 3 h post-mixing (lane 3) were used for RT–PCR. (Lane 4) Total genomic DNA of CU428 was also used for PCR. A 55-b intron is present in the genomic region, enabling the PCR products from genomic DNA (246 b) and cDNA (191 b) to be distinguished.