Figure 1. Cartoon of the constructs used.

The gene of GFPem was followed by a 30-residue linker rich in Gly and Ser, the 17-residue SecM AP or SecMstr AP and a stop codon. The mutated amino acids of SecMstr AP are shown in red letters. The control construct is missing the last 14 residues of the SecM moiety. All constructs could be linearized removing at the same time the stop codon.