Table 1. Activity and stalling efficiency of ribosomes expressing .

Techniques	Activity [%]	Stalling [%]
Co-precipitation	21.2 ± 3.8	93.6 ± 4.7
Two-colour coincidence detection	20.3 ± 7.7	84.7 ± 6.5
Two-colour colocalisation microscopy	17.4 ± 3.3	85.0 ± 5.9

Activity and stalling efficiency were measured with co-precipitation, TCCD (analysis of >28 000 ribosomes) and two-colour colocalisation microscopy (analysis of >12 500 ribosomes). For each technique, a minimum of three independent experiments was performed. The given errors are absolute standard deviations calculated from the repetitive measurements.