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. 2005 Jan 1;19(1):90–103. doi: 10.1101/gad.1267005

Figure 7.

Figure 7.

Hyperpolyadenylation of SSA4 mRNA in PAB1 null bypass strains. (A) Comparison of SSA4 mRNA poly(A) tail lengths in spb8Δ mutant strains. SSA4 mRNA RNase H assays were performed on 5 μg of total RNA from each strain either without (–) (lanes 1,3,5,7), or with (+) (lanes 2,4,6,8) 400 ng oligo (dT50). (Lanes 1,2) FY86 (WT). (Lanes 3,4) yAS2324 (spb8Δ). (Lanes 5,6) yAS2315 (spb8Δpab1Δ). (Lanes 7,8) yEDy201 (spb8Δpab1Δrrp6Δ). Lanes 1–4 represent a shorter exposure time as compared with lanes 5–8. (B) Comparison of SSA4 mRNA poly(A) tail lengths in pab1Δ bypass suppressor strains. SSA4 RNase H assays were performed on 5 μg of total RNA extracted from each strain after shifting to 42°C for 15 min. Strains used were yAS2315 (spb8Δpab1Δ, lane 3), EDy200 (rpl39Δpab1Δ, lane 5), EDy196 (pat1Δpab1Δ, lane 6), CMHy251 (rrp6Δpab1Δ, lane 7), FY86 (WT, lanes 3,8), and FY86 (WT) incubated with 400 ng oligo (dT50) prior to RNase digestion (lanes 2,9).