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. 2005 Jan 1;19(1):114–126. doi: 10.1101/gad.1255805

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Figure 5. — Degradation of Cdt1 does not require extensive DNA synthesis. (A) Sperm chromatin was incubated in LSS containing the indicated concentrations of aphidicolin. At various times, reactions were stopped and 1 μL of extract was blotted for Cdt1 and MCM3. Parallel reactions were carried out in the presence of [α-³²P]dATP and the percentage of input DNA replicated after 120 min is indicated. (B) Sperm chromatin was incubated in LSS containing 2 mg/mL methylated ubiquitin and the indicated concentrations of aphidicolin or 1 μM p27^Kip. Chromatin was isolated after 45 min and blotted for various replication proteins and RCC1 as a loading control.