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. 2017 Apr 24;7:46056. doi: 10.1038/srep46056

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Copyright © 2017, The Author(s)

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(A) Western blot analysis of cytochrome c after treatment of HepG2 cells with 1–3 at 0.25, 0.5, 1.0 μM for 24 h under the same experiment condition, respectively. (B) Densitometry analysis of cytochrome c band from part A. The relative expression of each band = (density of each band/density of actin band). Mean and SD values were obtained from three independent measurements. (C) RT-PCR to determine the expression of cytochrome c in the HepG2 cells treated with 1–3.