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Localization of YFP–ZapA requires FtsZ but not FtsA. YFP–ZapA and GFP–FtsQ localize in ftsZ84(Ts) or ftsA12(Ts) cells grown at 30°C (permissive temperature). When grown at 42°C (restrictive temperature), YFP–ZapA localizes in cells carrying the ftsA12(Ts) allele but not in those containing ftsZ84(Ts). GFP–FtsQ does not localize in either background at 42°C. The strains used are JOE95, JOE97, NWG128, and NWG129.
