Table 1.

Quantitative summary of localization data

	GFP fusion	FtsA	FtsKa	ZapA—FtsQ or FtsQ(wt)	Strain	Cells	Avg cell length (μm)	Fraction of cells w/rings	Rings/cell	Ring spacingb (μm/ring)
1	GFP—FtsL	+	-	ZapA—FtsQ	NWG252	65	22.1	0.95	2.3	9.5
2		+	-	FtsQ	NWG390	44	28.6	0.39	0.5	52.4
3		+	-	ZapA	NWG448	46	27.3	0.61	0.9	29.9
4		-	+	ZapA—FtsQ	NWG341	38	40.5	1.00	3.1	13.3
5		-	+	FtsQ	NWG418	36	41.6	0.56	0.9	48.3
6		-	+	ZapA	NWG447	20	33.0	0.8	1.2	28.7
7		-	-	ZapA—FtsQ	NWG259	77	49.3	1.00	4.2	11.8
8		-	-	FtsQ	NWG411	47	47.9	0.36	0.4	112.6
9	GFP—FtsI	+	-	ZapA—FtsQ	NWG277	53	25.1	0.94	2.3	10.8
10		+	-	FtsQ	NWG394	54	24.4	0.65	0.9	28.0
11		-	+	ZapA—FtsQ	NWG340	18	39.1	1.00	2.9	13.5
12		-	+	FtsQ	NWG417	32	45.0	0.69	0.8	55.4
13		-	-	ZapA—FtsQ	NWG299	30	51.2	1.00	3.5	14.5
14		-	-	FtsQ	NWG413	44	49.4	0.36	0.5	103.6
15	GFP—FtsN	+	-	ZapA—FtsQ	NWG257	207	7.0	0.85	1.0	7.1
16		+	-	FtsQ	NWG391	107	10.5	0.7	1.1	9.1
17		-	+	ZapA—FtsQ	NWG342	46	38.9	0.70	1.1	35.8
18		-	+	FtsQ	NWG419	27	46.3	0.85	1.4	32.9
19		-	-	ZapA—FtsQ	NWG269	41	38.8	0.8	1.2	33.1
20		-	-	FtsQ	NWG428	32	42.0	0.75	1.2	36.3
21	YFP—FtsK	-	+	ZapA—FtsQ	NWG352	28	40.0	1.00	4.7	8.5
22		-	+	FtsQ	NWG421	30	42.6	0.83	1.6	26.6
23		-	+	ZapA	NWG446	24	31.9	0.89	2.2	15.3
24	GFP—FtsQ	+	-	ZapA—FtsQ	NWG255	99	21.4	0.44	0.5	38.4
25		-	+	ZapA—FtsQ	NWG343	41	43.5	0.85	1.3	32.4
26		-	-	ZapA—FtsQ	NWG261	26	55.2	0.50	0.7	79.8
27		-	+	ZapA—FtsQ	NWG414	31	22.8	0.45	0.5	41.6
28		-	+++	ZapA—FtsQ	NWG415	50	21.1	0.9	1.9	11.0
29		-	+++	FtsQ	NWG423	23	41.9	0.87	1.6	26.8

^a+ indicates endogenous FtsK expression; +++ indicates endogenous FtsK plus expression from pBAD44.

^bRing spacing = total length (microns)/number rings; bold indicates positive localization score based on comparison with control cells (FtsQ or ZapA) and with published values of 8.1-14.5 μm/ring for localization of fts proteins in FtsN-depleted cells (Chen and Beckwith 2001).