Table 2.
Concentration (μg/ml) | Elastase activitya | Total proteaseb | Pyocyanin productionc | Chitinase activityd | EPS productione | Swarming motilityf | Biofilm formationg |
---|---|---|---|---|---|---|---|
Control | 0.181 ± 0.04 | 1.091 ± 0.04 | 4.4 ± 0.34 | 0.141 ± 0.019 | 1.110 ± 0.02 | 67 ± 2.5 | 0.406 ± 0.04 |
100 | 0.149 ± 0.03 (17.6) | 0.857 ± 0.03 (21.4) | 1.33 ± 0.33 (69.7)∗∗ | 0.110 ± 0.02 (21.9) | 0.678 ± 0.02 (38.9) | 50 ± 2.0 (25.2) | 0.256 ± 0.03 (36.9) |
200 | 0.110 ± 0.02 (39.2)∗ | 0.613 ± 0.03 (43.8)∗ | 0.87 ± 0.20 (80.2)∗∗ | 0.09 ± 0.01 (36.1) | 0.601 ± 0.02 (45.8) | 34 ± 2.0 (48.9)∗ | 0.210 ± 0.02 (48.2)∗ |
400 | 0.090 ± 0.02 (50.2)∗ | 0.547 ± 0.02 (49.8)∗ | 0.63 ± 0.03 (85.6)∗∗∗ | 0.081 ± 0.01 (42.5) | 0.552 ± 0.01 (50.2)∗ | 26 ± 1.5 (60.3)∗ | 0.179 ± 0.03 (55.9)∗ |
800 | 0.043 ± 0.02 (76.2)∗∗ | 0.479 ± 0.02 (56.0)∗ | 0.49 ± 0.02 (88.8)∗∗∗ | 0.063 ± 0.01 (55.3)∗ | 0.462 ± 0.02 (58.3)∗ | 17 ± 1.5 (73.7)∗∗ | 0.112 ± 0.02 (72.4)∗∗ |
Azithromycin (2 μg/ml) | 0.048 ± 0.01 | 0.389 ± 0.02 | 0.2 ± 0.009 | 0.04 ± 0.01 | 0.34 ± 0.02 | 21.6 ± 2.3 | 0.095 ± 0.01 |
aElastase activity is expressed as the absorbance at 495 nm. bTotal protease activity is expressed as the absorbance at 600 nm.cPyocyanin concentrations were expressed as micrograms of pyocyanin produced per μg of total protein. dChitinase activity is expressed as the absorbance at 570 nm. eEPS production is expressed as absorbance at 480 nm. fSwarming motility is expressed as diameter of swarm in mm. gBiofilm formation is expressed at 470 nm after incubation with crystal violet. Data represent mean and standard deviation of three independent experiments. Values in parentheses indicate percent reduction over control. ∗p ≤ 0.05, ∗∗p ≤ 0.005, ∗∗∗p ≤ 0.001.