. 2017 Apr 24;8:727. doi: 10.3389/fmicb.2017.00727

Table 2.

Effect of Mangifera indica on inhibition of quorum sensing (QS) regulated virulence factors in P. aeruginosa PAO1.

Concentration (μg/ml)	Elastase activity^a	Total protease^b	Pyocyanin production^c	Chitinase activity^d	EPS production^e	Swarming motility^f	Biofilm formation^g
Control	0.181 ± 0.04	1.091 ± 0.04	4.4 ± 0.34	0.141 ± 0.019	1.110 ± 0.02	67 ± 2.5	0.406 ± 0.04
100	0.149 ± 0.03 (17.6)	0.857 ± 0.03 (21.4)	1.33 ± 0.33 (69.7)^∗∗	0.110 ± 0.02 (21.9)	0.678 ± 0.02 (38.9)	50 ± 2.0 (25.2)	0.256 ± 0.03 (36.9)
200	0.110 ± 0.02 (39.2)^∗	0.613 ± 0.03 (43.8)^∗	0.87 ± 0.20 (80.2)^∗∗	0.09 ± 0.01 (36.1)	0.601 ± 0.02 (45.8)	34 ± 2.0 (48.9)^∗	0.210 ± 0.02 (48.2)^∗
400	0.090 ± 0.02 (50.2)^∗	0.547 ± 0.02 (49.8)^∗	0.63 ± 0.03 (85.6)^∗∗∗	0.081 ± 0.01 (42.5)	0.552 ± 0.01 (50.2)^∗	26 ± 1.5 (60.3)^∗	0.179 ± 0.03 (55.9)^∗
800	0.043 ± 0.02 (76.2)^∗∗	0.479 ± 0.02 (56.0)^∗	0.49 ± 0.02 (88.8)^∗∗∗	0.063 ± 0.01 (55.3)^∗	0.462 ± 0.02 (58.3)^∗	17 ± 1.5 (73.7)^∗∗	0.112 ± 0.02 (72.4)^∗∗
Azithromycin (2 μg/ml)	0.048 ± 0.01	0.389 ± 0.02	0.2 ± 0.009	0.04 ± 0.01	0.34 ± 0.02	21.6 ± 2.3	0.095 ± 0.01

^aElastase activity is expressed as the absorbance at 495 nm. ^bTotal protease activity is expressed as the absorbance at 600 nm_.^cPyocyanin concentrations were expressed as micrograms of pyocyanin produced per μg of total protein. ^dChitinase activity is expressed as the absorbance at 570 nm. ^eEPS production is expressed as absorbance at 480 nm. ^fSwarming motility is expressed as diameter of swarm in mm. ^gBiofilm formation is expressed at 470 nm after incubation with crystal violet. Data represent mean and standard deviation of three independent experiments. Values in parentheses indicate percent reduction over control. ^∗p ≤ 0.05, ^∗∗p ≤ 0.005, ^∗∗∗p ≤ 0.001.