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. 2017 Mar 30;7(1):37–44. doi: 10.4103/2045-9912.202908

Figure 2.

Figure 2

Menadione amplification of carbon monoxide (CO) production by cytochrome P450 reductase (CPR) in the presence or absence of heme oxygenase (HO).

Note: The open circles show the formation of CO when the reaction mixture contained 50 μM methemalbumin, 0.01 μM purified human NADPH-P450 reductase (recombinant; Becton Dickinson Canada Inc.), 0.7 μM full-length human HO-2 (FL-hHO-2) and various concentrations of menadione (MD) in 100 mM phosphate buffer (pH 7.4). The closed triangles show the formation of CO in the same reaction without FL-hHO-2 present. No CO production was detected in the absence of CPR. Bars represent SD, where bars are not shown the SD is smaller than the symbol; n = 4.