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. 2005 Jan 1;19(1):176–187. doi: 10.1101/gad.321305

Figure 1.

Figure 1.

The two FLPs are encoded by a single nuclear gene and generated through alternative splicing. (A) Alignment between C. reinhardtii Flu Like Proteins (FLPs) and A. thaliana FLU. The two FLPs differ only by 12 residues (indicated in bold). Identical residues and conservative replacements are highlighted in dark and light gray, respectively. The putative domains predicted by proteomic tools are indicated: chloroplast transit peptide (gray box, solid line); the two coiled-coil domains (striped boxes, dotted lines); hydrophobic domain (black box, dashed line), and the TPR domains (C-ter box, double lines). Gaps (—) were introduced to maximize the alignment. (B) PCR analyses performed with the primer set indicated in C and described in Materials and Methods on genomic DNA (lane 1), cDNA synthetized by RT–PCR from cells grown in continuous light (lane 2), l-FLP cDNA (lane 3), and s-FLP cDNA (lane 4). The unlabeled lanes contain size markers. (D) Immunoblot of total light-grown cells with antiserum raised against the C-terminal part of FLP (indicated with a bar in A).