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. 2005 Jan 1;19(1):176–187. doi: 10.1101/gad.321305

Figure 4.

Figure 4.

GST-pull-down assays to test the interactions of FLPs with the glutamyl-tRNA reductase (Glu-tRNA). (A) Different purified GST fusion proteins (l-FLP, s-FLP, and an N-terminal deletion construct of FLP [Δ-FLP]) were incubated with Chlamydomonas (C.r.) and Arabidopsis (A.t.) protein extracts. (B) The interactions were detected by immunoblotting of the bound fraction using anti-Glu-tRNA antibody and FLP antiserum, as control.