Fig. 2.

The suppressor1 mutation affects AtPH1. (A) Alignment of reads from the nns1 mutant to the Col-0 reference genome (TAIR10) at the AtPH1/At2g27900 locus. Paired reads are in blue; broken pairs are in red and green. The translation of the At2g27900.1 gene model is given under the nucleotide sequence. The asterisk denotes the mutation identified in nns1 converting Arg46 to His. (B) Amino acid sequence of AtPH1/At2g27900.1. The PH domain is highlighted in gray, and the PPBM is highlighted in red. (C) Alignment of the AtPH1 PH domain N terminus showing conservation to other PH domain from Oryza sativa (XP_015638090.1), Physcomitrella patens (XP_001766896.1), Dictyostelium discoideum (XP_643886.1), A. thaliana DRP2A (NP_172500.1), Homo sapiens PKB/AKT1 (NP_001014431.1) and DAPP1 (NP_055210.1), and Saccharomyces cerevisiae ScOSH2 (NP_010265.1). The background color reflects sequence conservation. (D) Wild-type (Ws), nramp3-1nramp4-1, nns1, and homozygous T3 nns1 lines constitutively expressing AtPH1-GFP were grown vertically for 10 d on ABIS medium without iron (−Fe). (E) Wild-type (Col), nramp3-2nramp4-2, and nramp3-2nramp4-2atph1-2 plants were grown vertically for 8 d on ABIS medium without iron (−Fe) or for 10 d on medium containing 30 µM CdCl₂ (+Cd). (Scale bars: 10 mm.)