Fig. 4.

AtPH1 colocalizes with a marker of the LE/MVB. nns1 plants expressing AtPH1-GFP were crossed with plants expressing the trans-Golgi marker ST-mRFP, the TGN/EE marker mRFP-SYP43, or the LE/MVB marker mRFP-ARA7. Colocalization of fluorescent markers was analyzed by confocal microscopy on root epidermal cells of F1 plants. On merged pictures the overlap of GFP (green) and mRFP (magenta) channels appears white. Cell contours are represented by dashed lines. The regions within the white outline are enlarged in the lower panels (magnification: 3×). Pearson (r_p) and Spearman (r_s) correlation coefficients as well as M1 (GFP) and M2 (mRFP) Manders overlap coefficients above threshold were calculated. (Scale bars: 10 µm.)