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The expression of AtPH1 under the control of the 2x35S promoter does not affect root growth under Fe-sufficient conditions. Wild-type (Ws), nramp3-1nramp4-1, nns1, and homozygous T3 nns1 lines constitutively expressing AtPH1-GFP under the control of the 2x35S promoter were grown vertically for 10 d on ABIS-agar medium containing 50 µM Fe-HBED (+Fe). This experiment was performed side-by-side with the experiment presented in Fig. 2D. (Scale bar: 10 mm.)
