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. 2017 Apr 3;114(16):E3354–E3363. doi: 10.1073/pnas.1702975114

Fig. S2.

Fig. S2.

Molecular characterization of the atph1 and atph2 mutants. (A and B) Structure of the AtPH1/At2g29700.1 (A) andAtPH2/At5g05710.1 (B) ORFs showing the localization of the mutations isolated in this study. The localization of the primers used for RT-PCR experiments is shown also. (C) The presence of AtPH1 and AtPH2 transcripts in different genetic backgrounds was analyzed by RT-PCR using AtPH1-for3/AtPH1-rev1 and AtPH2-for1/AtPH2-rev1 primers, respectively. The PCR products were analyzed on a 1.5% agarose gel with the GeneRuler 1 Kb Plus DNA ladder (Thermo Fisher Scientific).