Fig. S6.

Colocalization of AtPH1 with ARA6. Confocal images of the root elongation zone of transgenic F1 plants expressing AtPH1-GFP (A) or NRAMP1-GFP (B) and the LE/MVB marker mRFP-ARA6. Areas outlined in white on the left are enlarged on the right (magnification: 3×). On these merged images, the overlap of the GFP (green) and mRFP (magenta) channels appears in white. Dashed lines represent cell contours. Pearson (r_p) and Spearman (r_s) correlation coefficients as well as M1 (GFP) and M2 (mRFP) Manders overlap coefficients above threshold were calculated. (Scale bars: 10 µm.)