Fig. S7.

The nns1 mutant is not visibly affected in the biogenesis of ILVs. (A and B) Transmission electronic microscopy images of root cells from wild-type (Ws) (A) and the nns1 mutant (B). GA, Golgi apparatus; MVB, multivesicular bodies; V, vacuole. (Scale bars: 200 nm.) (C) The diameter of MVBs and the number of ILVs per MVB were quantified in Ws and nns1 strains. Results are shown as mean ± SD (Ws: 82 MVBs, 43 sections, two roots; nns1: 96 MVBs, 37 sections, two roots). Results were analyzed using a nonparametric Mann–Whitney test.