Fig. S8.

The expression of NRAMP1-GFP complements the Mn deficiency phenotype of nramp1. The nramp1-1 mutant (Col) was transformed with pMUBI83-NRAMP1. Among four independent lines showing complementation of nramp1-1 phenotype, the homozygous single-locus line #K is shown (two homozygous T3 progenies). Plants were grown vertically for 9 d on Hoagland-agar medium made with EDTA-washed agar containing 20 µM Fe-HBED and supplemented (+Mn) or not (−Mn) with 5 µM MnSO₄. (Scale bars: 10 mm.)