Fig. S9.

Effect of Concanamycin A on NRAMP1-GFP localization. Root epidermal cells of nramp3nramp4 and nns1 mutants expressing NRAMP1-GFP were imaged across vacuolar planes by spinning disk confocal microscopy. GPF fluorescence and transmitted light images (DIC) are displayed. Plants were treated with 2 µM Concanamycin A (ConcA) for 3 h before imaging or with DMSO (0.1%) as control. The arrows indicate the position of vacuolar structures in which GFP signal accumulates upon ConcA treatment. (Scale bar: 10 µm.)