Table S1.
Primers used in this study
| Target | Primer | Sequence | Mutant analysis |
| NRAMP3/At2g23150 | NRAMP3-for1 | GAAATACCGAGTCCAAGAAGC | To amplify NRAMP3 from Ws DNA. Use NRAMP3-for1 and LB102 for nramp3-1. |
| NRAMP3-rev1 | TCTCGTCTCTGGGACCTAACT | ||
| NRAMP3-for2 | TGTCCCAGAAAAACAAACAAAAC | To amplify NRAMP3 from Col DNA. Use NRAMP3-for2 and LBb1 for nramp3-2. | |
| NRAMP3-rev2 | TCCTGATTCCAACAAAAAGACC | ||
| NRAMP4/At5g67330 | NRAMP4-for1 | TTTGGTCAGACGAAACCCAGT | To amplify NRAMP4 from Ws DNA. Use NRAMP4-for1 and JL202 for nramp4-1. |
| NRAMP4-rev1 | TAAAACGACTTGGCAAACACC | ||
| NRAMP4-for2 | AGTCGTCACTTTAGTCGCAGC | To amplify NRAMP4 from Col DNA. Use NRAMP4-rev2 and LBa1 for nramp4-2. | |
| NRAMP4-rev2 | TAAAACGACTTGGCAAACACC | ||
| NRAMP1/ At1g80830 | NRAMP1-for1 | CCCATGATTAAACCACCAATG | To amplify NRAMP1 from Col DNA. Use NRAMP1-for1 and LBb1 for nramp1-1. |
| NRAMP1-rev1 | TCTCGCACTTCAAAAATACGG | ||
| AtPH1/At2g29700 | At2g29700-fwd | ACGCAGAGAGCCAGCATAAT | To amplify AtPH1 from Col DNA. Use At2g29700-rev2 and GABI-LB for atph1-2. |
| At2g29700-rev2 | TCCTCCTTCAGAAAACCCCTA | ||
| AtPH1-dCrev1 | CCTCGTTTGAGAACGAACCAT | Cut the At2g29700-fwd/AtPH1-dCrev1 PCR product with TaqI for atph1-1. | |
| AtPH1-for3 | GTCGTGGTGAGATTTTAG | To amplify AtPH1 transcript by RT-PCR. | |
| AtPH1-rev1 | CCACTCTTCTTTCTCCTTCTC | ||
| AtPH2/At5g05710 | At5g05710-fwd1 | TTTTGGGGCAAAAGATAAACA | To amplify AtPH2 from Col DNA. Use At5g05710-rev1 and LBa1 for atph2-1. |
| At5g05710-rev1 | GTCTTTGAACCAGAAGCGTTG | ||
| AtPH2-for1 | CTGTGGCGAGCAGTAATC | To amplify AtPH2 transcript by RT-PCR. | |
| AtPH2-rev1 | CCAATCTTCTTTCTCCTTCTCC | ||
| T-DNA | LB102 | GATGCACTCGAAATCAGCCAATTTTAGAC | |
| JL202 | CATTTTATAATAACGCTGCGGACATCTAC | ||
| LBa1 | TGGTTCACGTAGTGGGCCATCG | ||
| LBb1 | GCGTGGACCGCTTGCTGCAACT | ||
| GABI-LB | TATTGACCATCATACTCATTGC |