Fig. 1.

Experimental design. For this study, PCOS was induced in wild-type, global, neuron-specific, and granulosa cell-specific androgen receptor knockout mice by s.c. inserting dihydrotestosterone implants in the mice for 3 mo. Control mice were implanted with blank implants. Body weight, estrous cycling, blood pressure, fasting glucose, oral glucose tolerance, and insulin tolerance were assessed before collection of serum and tissues at 16 wk of age.