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. 2017 Mar 20;114(16):E3334–E3343. doi: 10.1073/pnas.1616467114

Fig. 4.

Fig. 4.

Global and neuron but not granulosa cell loss of AR signaling protects against the reduction in large antral follicle health in the PCOS mouse model. (A) Percentage of unhealthy small antral follicles per ovary, confirming DHT-induced increase in unhealthy small antral follicles in WT mice and showing no significant decrease in small antral follicle health in DHT-induced PCOS ARKO ovaries. Data are the mean ± SEM; n = 4 ovaries per genotype/treatment group. (B) Percentage of unhealthy large antral follicles per ovary, showing no significant decrease in large antral follicle health in DHT-induced PCOS ARKO or NeurARKO female mice. Data are the mean ± SEM; n = 4 ovaries per genotype/treatment group. (C) Average thickness of granulosa cell layer per follicle, displaying no reduction in granulosa cell-layer thickness in DHT-induced PCOS ARKO or GCARKO ovaries. Data are the mean ± SEM; n = 7 to 24 follicles per genotype/treatment group. (D) Average percentage of theca area per follicle, showing no significant increase in theca cell area in DHT-induced PCOS ARKO ovaries. Data are the mean ± SEM; n = 7 to 24 follicles per genotype/treatment group. (E) Histological sections representing the effects of DHT on granulosa cell-layer thickness (ii, double-ended arrow) and percentage of theca area (iv, white line) compared with controls (i and iii, respectively). G, genotype; ns, no significant difference; T, DHT treatment; *, significant difference. (Magnification: 10×.) (*P < 0.05, two-way ANOVA.)