Global loss of AR signaling protects against the reduction in adiponectin levels, adipocyte hypertrophy, and altered glucose homeostasis in the PCOS mouse model. (A) Serum levels of adiponectin, confirming DHT-induced suppression of serum adiponectin levels in WT mice and showing no significant reduction in adiponectin levels in DHT-induced PCOS ARKO female mice. Data are the mean ± SEM; n = 5 to 7 per genotype/treatment group. (B) Adipocyte size, showing no development of adipocyte hypertrophy in DHT-induced PCOS ARKO female mice. Data are the mean ± SEM; n = 3 sections per mouse, 3 mice per genotype/treatment group. (C) Histological sections of representative parametrial fat pads from each treatment group, showing no development of adipocyte hypertrophy in DHT-induced PCOS ARKO female mice. (D) Average fasting glucose levels showing no significant increase in glucose levels in DHT-induced PCOS ARKO female mice. Data are the mean ± SEM; n = 5 to 9 per genotype/treatment group. (E) Area under the curve (AUC) analysis of the oral glucose tolerance test, showing an overall effect of DHT treatment. Data are the mean ± SEM; n = 5 to 9 per genotype/treatment group. G, genotype; ns, no significant difference; T, DHT treatment; *, significant difference. (Magnification: 40×.) (*P < 0.05, two-way ANOVA.)