Characterization of global and tissue-specific ARKO mice. Representation of RT-PCR androgen receptor analyses using cDNA extracted from WT, ARKO, NeurWT, NeurARKO, GCWT, and GCARKO ovary, uterus, brain, and pituitary. The intact AR exon 3 PCR product is 288 bp long, whereas the excised AR exon 3 PCR product is 171 bp. Mouse β-actin was used as an internal control, with a PCR product of 431 bp.