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. 2016 Sep 16;95(37):e4809. doi: 10.1097/MD.0000000000004809

Figure 3.

Figure 3

Immunohistochemical staining for HLA-DR and WT-1 in the serial sections. (A) HLA-DR showed positive staining (black arrows) on the podocytes in the dn MN group. (B) WT-1 staining of the same glomerulus from A indicates the position of the podocytes (black arrows). (C) HLA-DR showed less staining on the podocytes in the IMN group than in the dn MN group. Black arrowheads indicate HLA-DR-negative podocytes. (D) WT-1 staining of the same glomerulus from A indicates the position of the podocytes (black arrowheads). A′, B′, C′, and D′ are magnified images of A, B, C, and D, respectively. (E) Negative control staining without primary antibody. Original magnification: ×400. dn MN = de novo membranous nephropathy, HLA = human leukocyte antigen, IMN = idiopathic membranous nephropathy, WT-1 = Wilms’ tumor-1.