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. 2017 Apr 24;10:106. doi: 10.1186/s13068-017-0790-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Freeze assay measurements for the cultivations of T. reesei, A. terreus, and P. verruculosum. Overall carbon release rate as a sum of glucose, cellobiose, and xylose carbon release rate. Colors indicate the distribution of sugars. Error bars represent standard deviation of technical triplicates. Assay conditions: 91 mM itaconic acid buffer (pH 3.7), 120 g L⁻¹ α-cellulose, incubation time 2 h, filling volume 1.1 in 2-mL tube, shaking frequency 900 rpm, shaking diameter 3 mm, and 30 °C