Table 2.
Mutation distribution of rpoB, katG, inhA promoter and gyrA genes in the smear-positive sputum specimens
| Drugs | Genes | Nucleotide change | Amino acid change | Number(%) | Number of phenotypically resistant strains |
|---|---|---|---|---|---|
| rifampicin | rpoB a | G(1546) → T | Asp(516) → Tyr | 2(1.59) | 1 |
| A(1547) → T | Asp(516) → Val | 1(1.59) | 1 | ||
| A(1547) → T;A(1552) → Gb | Asp(516) → Val; Asn(518) → Asp |
1(1.59) | 1 | ||
| AGA(1550–1552)deletionb | Frame shift | 1(1.59) | 1 | ||
| C(1576) → T | His(526) → Tyr | 2(2.18) | 2 | ||
| C(1576) → A | His(526) → Asn | 2(2.18) | 1 | ||
| C(1576) → G | His(526) → Asp | 1(1.59) | 1 | ||
| A(1577) → T | His(526) → Leu | 4(6.35) | 4 | ||
| A(1577) → G | His(526) → Arg | 1(1.59) | 1 | ||
| A(1577) → C | His(526) → Pro | 1(1.59) | 1 | ||
| C(1592) → T | Ser(531) → Leu | 44(68.25) | 44 | ||
| T(1598) → C | Leu(533) → Pro | 4(6.35) | 4 | ||
| C(1542) → T;ATGGAC(1543–1548)deletionb | Frame shift | 1(1.59) | 1 | ||
| isoniazid | katG | G(944) → C | Ser(315) → Thr | 40(95.24) | 40 |
| G(944) → A | Ser(315) → Asn | 2(4.76) | 2 | ||
| inhA promoter | T(−8) → C | 1(7.69) | 1 | ||
| C(−15) → T | 10(76.92) | 9 | |||
| G(−17) → T | 1(7.69) | 1 | |||
| C(−34) → Tc | 1(7.69) | 1 | |||
| ofloxacin | gyrA | C(269) → T | Ala(90) → Val | 5(14.29) | 4 |
| C(269) → G | Ala(90) → Gly | 2(5.72) | 1 | ||
| T(271) → C | Ser(91) → Pro | 4(11.43) | 4 | ||
| G(280) → T | Asp(94) → Tyr | 4(11.43) | 3 | ||
| G(280) → A | Asp(94) → Asn | 5(14.29) | 4 | ||
| A(281) → G | Asp(94) → Gly | 12(34.29) | 12 | ||
| A(281) → C | Asp(94) → Ala | 3(8.57) | 3 |
aFor rpoB gene, E coli codon numbering system is used
b, cthese mutations were not found in the TB Drug Resistance Mutation Database (https://tbdreamdb.ki.se/Info/)