Fig. 4.

Inhibition of HBsAg and HBeAg secretion by treatment with the herbal extracts on HepG2 2.2.15 cells. HBeAg and HBsAg producing HepG2 2.2.15 cells were treated for 72 h with same dosage (50 μg/ml) of ethanolic extracts of Dioscorea bulbifera, Lasiosphaera fenzlii, Paeonia suffruticosa, and Polygonum cuspidatum (labeled as DBE, LFE, PSE, and PCE). EtOH and ADV were used as negative and positive control, respectively. After treatments, levels of HBsAg (a) and HBeAg (b) in the culture media were determined by ELISA and presented as % of untreated control. The data represent the mean ± SD (n > 3). ***p < 0.001. EtOH 0.25% ethanol; ADV 10 μg/ml adefovir; H ₂ O ₂ 0.35%