Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strain | ||
| BW25113 (∆proC) | BW25113 with ∆proC:Km R; KmR | [32] |
| BW25113 (∆trpC) | BW25113 with ∆trpC:Km R; KmR | [32] |
| DH5α/λpir | Used for replication of pUC18T derivatives; λpir80dlacZ ΔM15 Δ(lacZYA-argG)U169 recA1 hsdr17 deoR thi-1 supE44 gyrA96 relA | [34] |
| Plasmid | ||
| pUC18T-mini-Tn7T-LAC-Gm | pUC18-based conditionally replicative delivery plasmid for mini-Tn7-LAC-Gm; ApR, GmR, mob+ | [33] |
| pUC18T-mini-Tn7T-LAC-Gm-egfp | pUC18T-mini-Tn7T-LAC-Gm containing egfp immediately downstream of Plac; ApR, GmR, mob+, egfp+ | this study |
| pUC18T-mini-Tn7T-LAC-Gm-echerry | pUC18T-mini-Tn7T-LAC-Gm containing echerry immediately downstream of Plac; ApR, GmR, mob+, echerry+ | this study |