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. 2017 Feb 1;158(5):945–961. doi: 10.1097/j.pain.0000000000000861

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Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 3. — Grpr-Cre–expressing neurons in the spinal cord are primarily excitatory. Immunohistological analysis of transverse sections of tdTomato;Grpr-Cre mouse spinal cord. (A-C) Adult lumbar spinal cord stained for IB4 (green), which marks inner lamina II. (D-F) Labeling of adult lumbar spinal cord with NeuN (green) revealed that most (97%) Grpr-Cre–positive cells are NeuN positive. (G-I) Postnatal (P4) spinal cord showing Grpr-Cre (G) and Viaat-egfp (H) expression in the spinal cord dorsal horn. (J-L) Adult dorsal spinal cord labeled for Grpr-Cre and Pax2 (green). Scale bars, 100 μm. The arrowheads highlight examples of coexpression.